Multiple crosslinked bio-inks for 3D microextrusion of tissue-like constructs and biodegradable thermoplastic elastomer for fuse deposition manufacturing
Biofabrication is providing scientists and clinicians the ability to produce engineered tissues with desired shapes, chemical and biological gradients. Typical resolutions achieved with extrusion based bioprinting are at the macroscopic level. However, for capturing the fibrillar nature of the extracellular matrix (ECM), it is necessary to arrange ECM components at smaller scales, down to the sub-micron and the molecular level.
In this project, we introduce a (bio)ink containing hyaluronan (HA) as tyramine derivative (THA) and collagen (Col). Similarly, to other connective tissues, in this (bio)ink Col is present in fibrillar form and HA as viscoelastic space filler. THA was enzymatically crosslinked under mild conditions allowing simultaneous Col fibrillogenesis, thus achieving a homogeneous distribution of Col fibrils within the viscoelastic HA-based matrix. THA-Col composite displayed synergistic properties in terms of storage modulus and shear-thinning, translating into good printability.
Shear-induced alignment of the Col fibrils along the printing direction was achieved and quantified via immunofluorescence and second harmonic generation (Figure). Cell-free and cell-laden constructs were printed and characterized, analyzing the influence of the controlled microscopic anisotropy on cell behavior and chondrogenic differentiation. THA-Col showed cell instructive properties modulating hMSC adhesion, morphology. Based on gene expression of cartilage/bone markers and matrix production, hMSCs embedded into the bioink displayed chondrogenic differentiation comparable or superior to standard pellet culture by means of proteoglycan production (Safranin O staining and proteoglycan quantification) as well as increase in cartilage related genes.
The possibility of printing matrix components with control over microscopic alignment brings
biofabrication one step closer to capturing the complexity of native tissues and the developed bioink can be integrated in the osteochondral implant of the OCD consortium.
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Palladino S, Schwab A, D'Este M, Copes F, Candiani G, Mantovani D. Innovative bioink from collagen and hyaluronic acid with tunable rheological and biological properties for cardiovascular 3D bioprinting. 2021 TERMIS world congress digital (oral)
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Malda J (Prof) and Levato R (PhD), The University Medical Center Utrecht, the Netherlands
Bastiaansen-Jenniskens YM (PhD), Narcisi R (PhD) and van Osch G (Prof), The University Medical Center Rotterdam, the Netherlands
Ho K (MD, PhD), Qin L (Prof, MD), Chinese University of Hong-Kong, Hong-Kong
Burdick J (PhD), Mauck R (Prof), the University of Pennsylvania, USA