Antibiofilm therapy using local application of bacteriophages (Antibio-LAB)

Background

The Antibio-LAB project aims to develop and asses a local application mode for bacteriophage therapy of difficult to treat, antibiotic resistant orthopaedic device-associated infections. The Antibio-LAB project aims to optimise an injectable and degradable thermo-responsive hyaluronan formulation for the delivery of these phages directly to the site of infection. Within this initial project period, several tests regarding loading phages into the thermo-responsive HA-pNIPAM hydrogel were performed. The release profile, kinetics and storage conditions of phages in the hydrogel were analysed. Overall, the thermo-responsive HA-pNIPAM hydrogel seems to be suitable for ISP-phage embedding, while retaining its thermo-responsive properties. Release of phage for the gelled HA-pNIPAM samples can be observed for 21 days. Hydrogels with looser polymer networks (I.e. lower polymer concentration) do not show a faster release of phage load, indicating that the diffusion of ISP-phage from the hydrogel is minimally hindered by the polymer network at all tested concentrations.

 


  • Publication

    Rotman SG, Sumrall E, Ziadlou R, Grijpma DW, Richards RG, Eglin D, Moriarty TF (2020) Local bacteriophage delivery for treatment and prevention of bacterial infections. Front Microbiol 11:538060. doi: 10.3389/fmicb.2020.538060.

  • Presentation

    Moriarty F, Rotman S. Local Application of Bacteriophage in biofilm-associated orthopaedic infections. The Bacteriophage Networking Meeting, 14 December 2019, Zürich, Switzerland (oral)

    Moriarty F. Optimal delivery method. 38th Annual Meeting of the European Bone and Joint Infection Society, 12-14 September 2019, Antwerpen, Belgium (oral)

  • Partner

    Lavigne R (MD), KU Leuven, Belgium

    Metsemakers W-J, UZ Leuven, Belgium

    Trampuz A (MD), Charité, Berlin, Germany

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