Antibiofilm therapy using local application of bacteriophages (Antibio-LAB)
Background
The Antibio-LAB project aims to develop and asses a local application mode for bacteriophage therapy of difficult to treat, antibiotic resistant orthopaedic device-associated infections. The Antibio-LAB project aims to optimise an injectable and degradable thermo-responsive hyaluronan formulation for the delivery of these phages directly to the site of infection. Within this initial project period, several tests regarding loading phages into the thermo-responsive HA-pNIPAM hydrogel were performed. The release profile, kinetics and storage conditions of phages in the hydrogel were analysed. Overall, the thermo-responsive HA-pNIPAM hydrogel seems to be suitable for ISP-phage embedding, while retaining its thermo-responsive properties. Release of phage for the gelled HA-pNIPAM samples can be observed for 21 days. Hydrogels with looser polymer networks (I.e. lower polymer concentration) do not show a faster release of phage load, indicating that the diffusion of ISP-phage from the hydrogel is minimally hindered by the polymer network at all tested concentrations.
Rotman SG, Sumrall E, Ziadlou R, Grijpma DW, Richards RG, Eglin D, Moriarty TF (2020) Local bacteriophage delivery for treatment and prevention of bacterial infections. Front Microbiol 11:538060. doi: 10.3389/fmicb.2020.538060.
Moriarty F, Rotman S. Local Application of Bacteriophage in biofilm-associated orthopaedic infections. The Bacteriophage Networking Meeting, 14 December 2019, Zürich, Switzerland (oral)
Moriarty F. Optimal delivery method. 38th Annual Meeting of the European Bone and Joint Infection Society, 12-14 September 2019, Antwerpen, Belgium (oral)
Lavigne R (MD), KU Leuven, Belgium
Metsemakers W-J, UZ Leuven, Belgium
Trampuz A (MD), Charité, Berlin, Germany