Currently, the translation of research from the lab to the clinic is not reliable due to an oversimplification of the in vitro models and limitations of animal testing. Most in vitro models provide static or oversimplified dynamic (e.g., only compression) environments over short-term tissue culture periods.
The overall objective is to develop a multi-axis dynamic in vitro system to mimic movement, with a focus on intervertebral discs (IVD), for long-term musculoskeletal tissue culture. This interdisciplinary project is a collaboration between CSEM (6-DOF bioreactor), ETH Zurich (biomechanics) and ARI (in vitro organ models).
In a biological and mechanical study a new sample holder for bovine IVDs with adjacent bone was validated according to bioreactor requirements for multiaxial loading and long-term IVD culture. During three weeks of culture under axial compression loading, the new model maintained the cell viability comparable to the standard model. When differently directed motions were applied, the holder-IVD interface with side screws resisted compression and torsion above reference values, and the combination of side and top screws resisted tension and bending at high values
Šećerović A, Ristaniemi A, Cui S, Li Z, Soubrier A, Alini M, Ferguson SJ, Weder G, Heub S, Ledroit D, Grad S. Towards the next generation of spine bioreactors: validation of an ex vivo intervertebral disc organ model and customized specimen holder for multiaxial loading. ACS Biomater Sci Eng. 2022 Aug 17. doi: 10.1021/acsbiomaterials.2c00330. Online ahead of print. PMID: 35977717
Ristaniemi A, Šećerović A, Dischl V, Crivelli F, Heub S, Ledroit D, Weder G, Grad S, Ferguson SJ. Physiological and degenerative loading of bovine intervertebral disc in a bioreactor: A finite element study of complex motions. J Mech Behav Biomed Mater. 2023;143:105900. https://doi.org/10.1016/j.jmbbm.2023.105900
Mürner M. Flexion, shear, and dynamic compression-induced IVD degeneration models for the evaluation of injectable biomaterial efficacy. 2023 ETH Zürich (S.J. Ferguson, S. Grad, A. Šećerović – MSc)
Ristaniemi A, Secerovic A, Ferguson SJ, Grad S. Viscoelastic characterization of bovine annulus fibrosus lamellae. 2022 WCB (oral)
Šećerović A, Ristaniemi A, Cui S, Li Z, Alini M, Crivelli F, Heub S, Weder G, Ferguson SJ, Ledroit D, Grad S. Development and validation of the new generation of bioreactors for long-term intervertebral disc organ culture under multiaxial loading. 2023 ORS (poster)
Ferguson SJ (Prof), ETH Zürich, Switzerland
Weder G (Dr), CSEM Neuchâtel, Switzerland
Heub S (Dr), CSEM Neuchâtel, Switzerland