3D printed constructs for osteochondral defect repair (MultiBio-Ink)

ARI Exploratory Research
D Eglin, M D'Este, M Alini


Osteochondral defects are still a major clinical challenge. They represent a large societal burden as they limit employment and impede daily life activities of millions of Europeans. Moreover, these injuries often lead to further degeneration of the joint, into a disabling disease known as osteoarthritis (OA). The defect bridges two major tissue types (cartilage and bone) that also have zonal structures within and specific healing capacities. Additionally, the cartilaginous surface must follow the patient specific contour of the surrounding tissue to avoid arthritic changes. In this project we bring together expertise in biomaterials and bioprinting to develop an additive manufacturing approach to produce constructs with precisely controlled internal architecture and the potential for patient specific implants. We are systematically investigating how to create collagen fibrils with controlled orientation within a hyaluronic acid-based matrix, thereby imitating the natural tissue composition. The possibility of printing matrix components with control over microscopic alignment brings biofabrication one step closer to capturing the complexity in animal tissues, opening new possibilities for clinical translation.


  • Publication

    Schwab A, Levato R,  D’Este M, Piluso S, Eglin D,  Malda J (2020) Printability and shape fidelity of bioinks in 3D bioprinting. Chemical Reviews 120 (19), 11028-11055.

    Schwab A, Hélary C, Richards RG, Alini M, Eglin D, D'Este M (2020) Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell migration and alignment. Materials Today Bio, 100058.

    Schwab A, Alini M, Eglin D,  D’Este M. 3D Drucken von Kollagenfibrillen mit Kontrolle über deren Orientierung in einer Hyaluronsäure Matrix als biomimetisches Knorpelimplantat. Zeitschrift für Orthopädie und Unfallchirurgie 158 (S 01), DKOU20-1036. DKOU 2020 (abstract)./p> Schwab A, Staubli F, Alini M, Eglin D, D’Este M. Einfluss der anisotropen Faserausrichtung und des Kollagen Typs und auf das chondrogene Differenzierungsverhalten. Zeitschrift für Orthopädie und Unfallchirurgie 158 (S 01). DKOU 2020 (abstract).

    Zheng Z, Eglin D, Alini M, Richards RG, Qin L, Lai Y. Visible light-induced 3D bioprinting technologies and corresponding bioink materials for tissue engineering: A review. Engineering. 2020;epub Sep 20.

    Zhou L, van Osch GJVM, Malda J, Stoddart MJ, Lai Y, Richards RG, Ki-Wai Ho K, Qin L. Innovative Tissue-Engineered Strategies for Osteochondral Defect Repair and Regeneration: Current Progress and Challenges. Adv Healthc Mater. 2020;epub Oct 26:e2001008.

    Xu Y, Yin H, Chu J, Eglin D, Serra T, Docheva D.  An anisotropic nanocomposite hydrogel guides aligned orientation and enhances tenogenesis of human tendon stem/progenitor cells.  Biomater Sci. 2021;epub Feb 12 https://doi.org/10.1039/D0BM01127D
  • Presentation
    Schwab A, Eglin D, Alini M,  D'Este M.Control of microarchitecture within biomaterial ink through 3D printing to guide cell migration. WBC 2020.

  • Partner

    Burdick J, University of Pennsylvania
    Mauck R, University of Pennsylvania
    Levato R, University Medical Center Utrecht
    Malda J, University Medical Center Utrecht
    Ho K, Chinese University of Hong Kong
    Qin L, Chinese University of Hong Kong
    van Osch GJVM, University Medical Center Rotterdam
    Stoddart M, AO Research Institute Davos

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