It has become increasingly evident that the gut microbiota plays a crucial role in many diseases, including bone-associated morbidities, such as osteoporosis, and possibly also fracture healing.
In this project, we are investigating the impact of the gut microbiota on bone in health and during a fracture related infection.
One way the gut microbiota interacts with bone is through production of metabolites, especially short-chain fatty acids (SCFAs). Butyrate is one class of SCFAs and we found, that butyrate (C4) inhibits murine, as well as human osteoclast formation in vitro. We performed RNA sequencing of osteoclast precursors cells treated with butyrate to identify differentially expressed genes, which may play a role in the butyrate mediated inhibition of osteoclastogenesis (Figure 1). In particular, we are focusing on genes involved in osteoclast formation (Figure 2).
Wallimann A, Thompson K, Stanic B, Akdis C, O'Mahony L, Moriarty F. "The influence of microbial-derived metabolites on bone health" at SBMS Summer School 16-17 May 2019 Bern and at MIM retreat 29-31 August, Grindelwald, Switzerland. (Oral)
Wallimann A, Thompson K, Stanic B, Akdis C, O'Mahony L, Moriarty F. "The influence of microbial-derived metabolites on bone health" at WIRM 3-6 April 2019 Davos, Switzerland, eCM conference 26-28 June 2019 Davos, Switzerland, SSM Annual Meeting 3-4 September 2019 Zurich, Switzerland and EAACI Winter School 23-26 January 2020, Chamonix, France.
Wallimann A, Thompson K, Stanic B, Akdis C, O'Mahony L, Moriarty F. Poster award for an outstanding poster presentation at EAACI Winter School 23-26 January 2020, Chamonix, France.
Liam O'Mahony, University College Cork, Ireland